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dap siliques  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dap siliques
    Dap Siliques, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dap+siliques/DAPTOMYCIN/pmc12447430-355-9-57
    Average 95 stars, based on 1 article reviews
    dap siliques - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Co-Immunoprecipitation Assay:

    Article Title: A bHLH7–PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana
    Article Snippet: The fluorescence signals were then detected using a confocal laser scanning microscope (Leica TCS SP8 SR, Germany). .. For the CoIP assay, total proteins were extracted from 12-DAP siliques collected from the transgenic tagging lines with extraction buffer (50 m M Tris–HCl, 150 m M NaCl, 5 m M EDTA, 1% [v/v] NP-40, 10% [v/v] glycerol, 1 m M PMSF, 5 m M DTT, and 1× protease inhibitor cocktail) and immunoprecipitated at 4°C overnight using Pierce anti-HA magnetic beads (Thermo Fisher, USA). ..

    Transgenic Assay:

    Article Title: A bHLH7–PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana
    Article Snippet: The fluorescence signals were then detected using a confocal laser scanning microscope (Leica TCS SP8 SR, Germany). .. For the CoIP assay, total proteins were extracted from 12-DAP siliques collected from the transgenic tagging lines with extraction buffer (50 m M Tris–HCl, 150 m M NaCl, 5 m M EDTA, 1% [v/v] NP-40, 10% [v/v] glycerol, 1 m M PMSF, 5 m M DTT, and 1× protease inhibitor cocktail) and immunoprecipitated at 4°C overnight using Pierce anti-HA magnetic beads (Thermo Fisher, USA). ..

    Extraction:

    Article Title: A bHLH7–PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana
    Article Snippet: The fluorescence signals were then detected using a confocal laser scanning microscope (Leica TCS SP8 SR, Germany). .. For the CoIP assay, total proteins were extracted from 12-DAP siliques collected from the transgenic tagging lines with extraction buffer (50 m M Tris–HCl, 150 m M NaCl, 5 m M EDTA, 1% [v/v] NP-40, 10% [v/v] glycerol, 1 m M PMSF, 5 m M DTT, and 1× protease inhibitor cocktail) and immunoprecipitated at 4°C overnight using Pierce anti-HA magnetic beads (Thermo Fisher, USA). ..

    Protease Inhibitor:

    Article Title: A bHLH7–PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana
    Article Snippet: The fluorescence signals were then detected using a confocal laser scanning microscope (Leica TCS SP8 SR, Germany). .. For the CoIP assay, total proteins were extracted from 12-DAP siliques collected from the transgenic tagging lines with extraction buffer (50 m M Tris–HCl, 150 m M NaCl, 5 m M EDTA, 1% [v/v] NP-40, 10% [v/v] glycerol, 1 m M PMSF, 5 m M DTT, and 1× protease inhibitor cocktail) and immunoprecipitated at 4°C overnight using Pierce anti-HA magnetic beads (Thermo Fisher, USA). ..

    Immunoprecipitation:

    Article Title: A bHLH7–PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana
    Article Snippet: The fluorescence signals were then detected using a confocal laser scanning microscope (Leica TCS SP8 SR, Germany). .. For the CoIP assay, total proteins were extracted from 12-DAP siliques collected from the transgenic tagging lines with extraction buffer (50 m M Tris–HCl, 150 m M NaCl, 5 m M EDTA, 1% [v/v] NP-40, 10% [v/v] glycerol, 1 m M PMSF, 5 m M DTT, and 1× protease inhibitor cocktail) and immunoprecipitated at 4°C overnight using Pierce anti-HA magnetic beads (Thermo Fisher, USA). ..

    Magnetic Beads:

    Article Title: A bHLH7–PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana
    Article Snippet: The fluorescence signals were then detected using a confocal laser scanning microscope (Leica TCS SP8 SR, Germany). .. For the CoIP assay, total proteins were extracted from 12-DAP siliques collected from the transgenic tagging lines with extraction buffer (50 m M Tris–HCl, 150 m M NaCl, 5 m M EDTA, 1% [v/v] NP-40, 10% [v/v] glycerol, 1 m M PMSF, 5 m M DTT, and 1× protease inhibitor cocktail) and immunoprecipitated at 4°C overnight using Pierce anti-HA magnetic beads (Thermo Fisher, USA). ..



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